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OBJECTIVE: To investigate the role and mechanism of the endoplasmic reticulum stress(ERS) pathway of apoptosis mediated by inositol-requiring enzyme-1(IRE1) in the intervention of silicosis fibrosis in rats using polyguanylic acid(PolyG).METHODS: The specific pathogen free adult male SD rats were randomly divided into control group(24rats),silicosis model group(24 rats),PolyG intervention group(16 rats) and PolyG treatment group(16 rats).The silicosis fibrosis rat model was constructed using the single inhalable intratracheal instillation method.The rats in the control group were injected with 1 mL of 0.9% sodium chloride solution.The other 3 groups were given 1 mL of silica suspension at 50.0 g/L mass concentration.The rats in PolyG intervention group on the day of model construction and rats in PolyG treatment group on the 28 th day after model construction were all given PolyG with 2.5 mg/kg body weight by one time tail vein intravenous injection.Eight rats in the PolyG intervention group and PolyG treatment group were sacrificed respectively on day 28 and day 56 after injection.The pathological changes of lung tissue in each group were observed.The expression of glucose regulated protein-78(GRP78),IRE1,CCAAT/enhancer-binding protein homologous protein(CHOP),Casepase-3,Casepase-12,type Ⅰ collagen and type Ⅲ collagen in lung tissue was detected by the Western blot.RESULTS: The histopathology examination results showed that the structure of lung tissue in control group was normal.The alveolar structure of the lung tissue of the silicosis model group was severe,and the fibrous nodules and a large amount of collagen deposition appeared.The silicosis nodules and collagen deposition in PolyG intervention group and PolyG treatment group were less than those in silicosis model group.The expression of GRP78,IRE1,CHOP,Casepase-3,Casepase-12,type Ⅰ collagen and type Ⅲ collagen in silicosis model group was higher than that of control group(P <0.05).The expression of the above 7 proteins in the PolyG intervention group and PolyG treatment group was lower than that of silicosis model group(P<0.05),higher than that of control group(P<0.05),except IRE1 and CHOP in PolyG intervention group.On day 56 after model construction,the expression of GRP78,IRE1,Casepase-3,Casepase-12,typeⅠ collagen and type Ⅲ collagen in PolyG intervention group were lower than that of PolyG treatment group(P<0.05).CONCLUSION: The unfolded protein response of ERS mediated by IRE1 may participate in the process of PolyG the intervention on silicosis fibrosis in rats.PolyG can effectively prevent and treat silicosis fibrosis.Prophylactic administration is recommended.
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ObjectiveTo observe the level of transforming growth factor-β1 ( TGF-31 ) in lung tissue of mice with acute lung injury induced by bleomycin. MethodsSixty Kunming male mice were randomly (random number) divided into sham operation group, model group, curcumin high dose group,medium dose group and low dose group (n =12 in each group). The models of acute lung injury were made by intratracheal instillation of bleomycin for once in the mice of model group and curcumin treated groups,and equal volume of saline was used in mice of sham operation group instead of bleomycin. Curcumin was given in doses of 200 mg· kg-1 · d-1 , 100 mg· kg-1 · d-1and 50 mg · kg-1 · d-1 to the mice of high dose group, medium dose group and low dose group, respectively 24 hours after modeling until the day before sacrifice, and the mice of sham operation group and model group were fed with equal volume of saline instead. Six mice of each group were sacrificed on the 3rd and 7th day separately for detecting lung wet to dry ratio (W/D), pathological changes of lung tissue stained with HE, and level of the transforming growth factor-β1 in the lung tissues stained with immunohistochemistry in mice of each group. Data were analyzed to express as mean ± standard deviation. -PSS11.5 statistical package was used for analysis. The comparison of the mean diversity carried out by using single factor analysis of variance. Results Morphological changes in lung tissue observed under light microscope showed that obvious morphological abnormalities,alveolar edema fluid and inflammatory cells were not found in mice of sham operation group, and lung capillaries dilated, widened alveolar septum, edema fluid and inflammatory cells infiltration in the alveolar in lung tissue were seen in mice of model group, and different degrees of pathological changes were found in mice of three curcumin treated groups, but those changes were less severe than those in mice of model group. Lung wet to dry ratio (W/D) was significantly higher in mice of model group than that in mice of sham group ( P ＜ 0. 01 ), but that in mice of three curcumin treated groups was significantly lower than that in mice of model group ( P ＜ 0. 01 ). Immunohistochemistry showed low level of TGF-β1 in lung tissue of mice in sham operation group, and the level of TGF-β1 in lung tissue of mice in model group was significantly higher than that in sham operation group ( P ＜ 0. 01 ). The level of TGF-β1 in lung tissue of mice in three curcumin treated groups was significantly lower than that in mice of model group ( P ＜ 0. 01 ),but that was still higher than that in sham group ( P ＜ 0. 01 ). ConclusionsCurcumin can inhibit the TGF-β1 in lung tissue and play a protective effect on acute lung injury induced by bleomycin, reducing the degree of lung injury. The protective mechanism may be associated with the inhibition of TGF-β1 in lung tissue.
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Objective To evaluate the correlation of the diagnostic chance of maternal pulmonary tuberculosis and the prognosis of their children. Methods One hundred and fifty-six patients with maternal pulmonary tuberculosis were analyzed,which were assigned to two groups based on the diagnostic chance: pregnant group ( 60 cases) and postpartum group ( 96 cases). Living quality of their children was counted, and viewed in parallel with children (98 cases,control group) of healthy mother at the same time. Results The morbidity in postpartum group was 69.79% (67/96),significantly higher than that in pregnant group [l3.33%(8/60)](P<0.05) and control group [4.08%(4/98)](P<0.05). The mortality in postpartum group was 8.33% (8/96), significantly higher than that in pregnant group (0)(P< 0.05) and control group (0)(P< 0.05).The morbidity and mortality in pregnant group were not significant difference compared with control group(P > 0.05 ). Conclusion Diagnosis and treatment before the parturition of maternal pulmonary tuberculosis can reduce the morbidity and mortality of the children and improve their life quality.
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Objective:To investigate the numbers and distributions of dendritic cells and the expressive intensity of CD1c,S 100 in different human ovarian carcinoma tissues,and to discuss the relationship between them and the mechanism of development.The study can provide the experimental date of biological therapy of ovarian carcinoma.Methods:The samples of 31 patients with ovarian carcinoma were analyzed by ABC immunocytochemistry and computer imaging techniques.Results:The results showed that the number of positive dendritic cells in ovarian carcinoma reduced greatly with significant and the expressive intensity of CD1c,S 100 was weaker in ovarian carcinoma than in normal ovarian tissues.Conclusion:The total expression of dendritic cells phenotypic antigens was reduced in ovarian epithelial carcinoma,which may play an important role in initiating immunity against ovarian carcinoma.